The application of a real time Polymerase chain reaction (PCR) system in the field of molecular diagnostics it has become a standard method for identifying nucleic acids from collected microbial samples. Real-time PCR (also known as RT-PCR) systems are sensitive, fast, accurate, and minimize the risk of contamination while used for various research procedures, such as gene expression detection and analysis, quantitative genotyping, early diagnosis of diseases. , forensic, etc. Since the introduction of RT-PCR, the technology is improving with every medical innovation. The COVID-19 pandemic has put RT-PCR in the spotlight, as it is considered the most accurate and efficient test method for the detection of the SARS-CoV-2 virus. The use of RT-PCR has expanded greatly for testing the new coronavirus, and demand is likely to grow due to the increasing incidence of infectious diseases.

Recently, Bio-Rad Laboratories introduced the company’s next generation of CFX real-time PCR systems, CFX Opus 96 and CFX Opus 384, which offers improved connectivity and high performance. The sleek and modern CFX systems present the next revolution in qPCR with their easy-to-use interface and advanced connectivity options. Both RT-PCR systems are ideal for basic and translational research, genomic testing, quality control, process development, infectious disease detection, and pathogen detection. Along with the expansion of Bio-Rad’s CFX product portfolio, the company also launched a cloud-based instrument connectivity and data management system. Verve.

Consolidating a precise optical shuttle system and improved thermal performance, the new CFX systems are designed to provide more consistent data than ever. Advanced RT-PCR systems are capable of independent operations, making them indispensable as life research tools for diagnostic interventions. The systems show excellent compatibility with Ethernet and Wi-Fi and therefore allow completely wireless operations and data management. The user can transfer the retrieved data by connecting the CFX systems with a PC or by direct data transfer to a USB device. At network-connected locations, the CFX Opus system can be programmed to send data and notifications directly to your email or local drive, and thus the updated systems provide an efficient experience for RT-PCR users.

CFX Opus 96 from Bio-Rad Laboratories has five color detection formats while CFX Opus 384 RT-PCR provides four color detection formats. The systems’ modern, responsive touchscreen is responsive even with double gloves and is more intuitive than previous generation CFX systems. However, the uniform optical system does not have regular calibration requirements, saving time and effort. In addition, the block design of the products offers close thermal uniformity during real-time PCR experiments.

Shuttle Optics Technology

The recently released CFX Opus systems retain many features of the previous generation models that researchers and scientists liked. Both systems have been built on the same property optical design, employed in the company’s CFX product portfolio, however, the latter are easy to configure and install due to enhanced capabilities and flexible connectivity options. The device manufacturer has endeavored to make the design of the CFX system sleek and modern, transcendent from a clamshell lid to a retractable version, allowing the RT-PCR to be used in more spaces than before. . Despite the design modifications, the updated system looks somewhat familiar to the older systems, but they are more convenient.

CXR Opus systems incorporate shuttle optics technology It uses the same excitation detector pair as other systems in Bio-Rad’s RT-PCR portfolio. In newer systems, moving the optics across the plate eliminates the possibility of variability that can result from detectors. In addition, the technology reduces the need for detector angle correction arising from a single fixed camera. Device consistency is enhanced by significant development in thermal performance, providing ± 0.3 ° C uniformity and ± 0.2 ° C accuracy. Both shuttle optics technology and thermal gradient of High performers work brilliantly together and produce consistent, reliable results.

CFX Maestro software

CFX systems come with an updated and complete CFX Maestro software, which is easy to use and learn. The software enables users to perform statistical analysis of gene expressions and find solutions to complex biological questions. Furthermore, user can perform analysis without exporting recovered data to PC with the help of Maestro software. The software also consists of a Laboratory Information Management System (LIMS) integration, an application programming interface (API), and other features for more advanced users.

BR.io cloud platform

Bio-Rad Laboratories has expanded the family of life sciences tools with the addition of BR.io cloud platform, which provides users with a simplified qPCR experience. The cloud connectivity platform built into CRX Opus systems provides a robust solution to meet customer needs in the expanding field of genomic research and diagnostics. By leveraging BR.io, users can retrieve remote data with any computer connected to the Internet, as well as monitor the status of registered instruments, so cloud connectivity ensures efficiency and provides greater versatility in operations. You can connect CFX Opus systems to the BR.io cloud platform via an Ethernet connection or with simple Wi-Fi connectivity and access the local network file drives that your institution has granted access to. Thus, BR.io provides seamless data transfer, allows users to access data from anywhere from the cloud, design experiments, and analyze data.

Latest additions to RT-PCR systems

Azure Cielo 3 real-time qPCR system

Launched in June 2020, the Azure Cielo real-time PCR system is a 96-well qPCR machine dedicated to the genomics workflow. The new RT-PCR system offers high throughput and provides an intuitive workflow due to its innovative optical design. The device ensures sensitivity and reproducibility with an optimal excitation reaction using single-well excitation and emission. The system has a 10.3 “touch screen interface that facilitates configuration and status verification. Data from the compact device can be retrieved via USB, Ethernet, or Wi-Fi in a connected environment. The RT-PCR device comes with Azure Sky Manager Software It offers a comprehensive workflow solution and enables accurate analysis. Azure Cielo qPCR is an easy-to-use, accurate instrument that provides good reproducibility and improves investigator productivity by automating sample extraction, amplification, and detection on a single cartridge.

Palm PCR S1

Ahram Biosystem has developed a ultra-fast mobile real-time PCR system which allows one to instantly complete PCR tests anywhere and anytime without electricity or communication. A brilliant innovation in RT-PCR systems, the Palm PCR S1 is seen as the future of biotechnology. The mobile device is extremely efficient and convenient, which can deliver results in less than 12 minutes with high precision even with a single copy target concentration. Developed with convection PCR technology, the ultra-fast system has a maximum of six real-time channels available, so the full capacity of the device can be achieved without detectable crosstalk. The standalone device consists of a lithium battery that allows four hours of continuous operation and can operate in temperatures up to 40 degrees Celsius. The device can be easily connected with a PC via Bluetooth and Wi-Fi.

conclusion

Recognizing the importance of molecular diagnostics for the detection of pathogens responsible for infectious diseases, many biotech companies are devising innovative RT-PCR systems. Standardization and quality control remain some of the main limitations of diagnostic PCR, but new methodologies and technologies use artificial intelligence to reduce the need for human participation. PCR diagnostics are likely to have a major impact on the personalization of healthcare and drug development in the years to come.

According to TechSci Research Report on “Global dPCR and qPCR Products Market By technology (quantitative, digital), by product (consumables and reagents, instruments, software and services), by application (clinical (pathogen tests, blood tests, oncology tests, others), research, forensic, others), by end user (Hospitals and diagnostic center, research laboratories and academic institute, pharmaceutical and biotechnology companies, clinical research organizations, forensic laboratories), by region, forecast and opportunities, 2025 “, it is estimated that the global market for dPCR and qPCR will register a significant CAGR due to the increasing incidence of infectious diseases, as well as an increasing application of biomarker profiles for disease diagnosis. In addition, the introduction of technological advances in PCR technologies and the high costs of PCR devices. Advanced PCR could hamper dPCR and qPCR market growth.

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